Isolation and characterization of the haemolymph lipoprotein of desert locust, Schistocerca gregaria Forskal. |
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Authors: | S K Patyal A Nath |
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Affiliation: | Dept. of Entomology and Apiculture, Dr. Y S Parmar University of Horticulture & Forestry, Solan. |
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Abstract: | A yellow lipoprotein was isolated and purified from haemolymph of desert locust, Schistocerca gregaria Forskal, by precipitation at low ionic concentration and by gel filtration. Sephadex G-200 resolved haemolymph into five protein peaks. First peak was of lipoprotein with molecular weight of 501,000 +/- 7000 (n = 3) and on electrophoresis, it separated into three bands. Maximum lipids from lipoprotein could be extracted with chloroform: methanol solvent system and were 52% by weight. |
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