Electrocoagulation as a clarifiation and pre-purification step in plant based bioprocesses |
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Authors: | Goran Robić Everson Alves Miranda |
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Institution: | (1) Centro Ricerche Casaccia, Via Anguillarese 301, I-00123 Rome, Italy;(2) Centro Ricerche Trisaia, SS 106 Ionica, I-75026 Rotondella (Matera), Italy |
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Abstract: | Electrocoagulation is a technique that has been applied to water and wastewater treatment. We propose here extension of this
technique to the field of plant downstream processing (DSP), where plants are used as a bioreactors for recombinant protein
production. The main problem in plant-based bioreactors is the presence of chlorophyll and phenolic compounds in plant extracts,
which tend to precipitate and denature proteins. Their removal from the extracts is essential, since they have a large impact
on the DSP performance. In the present work we studied the application of an electrocoagulation based technique to clarify
tobacco leaf extracts by removing chlorophyll and phenolic compounds. By manipulating the pH of electrocoagulation, 90% of
chlorophyll, 65% of phenolic compounds, and only 23% of total protein were removed from the extract. The process preferentially
removes proteins with more acidic pI (below 6.03) and the pH of the process differentially effects removal of acidic and basic
proteins. |
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