Development and activity of Glomus intraradices as affected by co-existence with Glomus claroideum in one root system |
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Authors: | Martina Janoušková Pascale Seddas Libor Mrnka Diederik van Tuinen Anna Dvořáčková Marie Tollot Vivienne Gianinazzi-Pearson Miroslav Vosátka Armelle Gollotte |
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Institution: | (1) Institute of Botany, Academy of Sciences of the Czech Republic, 252 43 Průhonice, Czech Republic;(2) UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 21065 Dijon Cedex, France |
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Abstract: | The co-existence of two arbuscular mycorrhizal fungal (AMF) species, Glomus intraradices and Glomus claroideum, in the root systems of plants was investigated in a greenhouse experiment aimed at reconstructing interactions during an
early stage of primary succession on a coal-mine spoil bank in Central Europe. Two plant species, Tripleurospermum inodorum and Calamagrostis epigejos, were inoculated either with one or both AMF species. Fungal development, determined by trypan blue and alkaline phosphatase
staining as well as by PCR amplification of rRNA genes with species-specific primers, and the expression of five genes with
different metabolic functions in the intraradical structures of G. intraradices were followed after 6 and 9 weeks of cultivation. The two AMF closely co-existed in the root systems of both plants possibly
through similar colonisation rates and competitivity. Inoculation with the two fungi, however, did not bring any additional
benefit to the host plants in comparison with single inoculation; moreover, plant growth depression observed after inoculation
with G. claroideum persisted also in mixed inoculation. The expression of all the assayed G. intraradices genes was affected either by host plant or by co-inoculation with G. claroideum. The effects of both factors depended on the time of sampling, which underlines the importance of addressing this topic in
time-course studies. |
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Keywords: | Alkaline phosphatase Arbuscular mycorrhiza Gene expression Intraradical mycelium Nested PCR |
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