| 猪瘟病毒野毒株持续感染细胞模型的稳定性研究 |
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| 引用本文： | 许振辉,应松成,沈超,屈三甫,郑从义,王在时.猪瘟病毒野毒株持续感染细胞模型的稳定性研究[J].Virologica Sinica,2003,18(3):271-274. |
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| 作者姓名： | 许振辉 应松成 沈超 屈三甫 郑从义 王在时 |
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| 作者单位： | [1]武汉大学生命科学学院，湖北武汉430072 [2]中国兽药监察所，北京100081 |
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| 基金项目： | 国家重点基础研究发展规划项目“973”(G1999011904) |
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| 摘 要： | 以本实验室建立的CSFV39-PKl5持续感染细胞模型为实验材料，综合运用免疫荧光、RT-PCR、流式细胞仪，对其稳定性进行研究。实验结果均表明，该细胞模型有着良好的稳定性。即使在连续传至128代的CSFV39-PKl5传代细胞中，CSFV仍持续存在：呈免疫荧光抗体反应阳性和RT-PCR检测阳性。同时，该细胞与正常的PK-15细胞相比，细胞周期无显著差异。通过同段序列的同源性比较，发现CSFV39与CSFV石门株的同源性最高，达99．02％。
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| 关 键 词： | 猪瘟病毒 野毒株 稳定性 细胞模型 持续感染 细胞周期 |
A Research of The Stability Based on A Cell Model Persistently Infected By A Field-isolated Virulent Strain of CSFV |
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| XU zhen-hui,YING Song-cheng,SHEN Chao,QU San-pu,ZHENG Cong-yi.A Research of The Stability Based on A Cell Model Persistently Infected By A Field-isolated Virulent Strain of CSFV[J].中国病毒学(英文版),2003,18(3):271-274. |
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| Authors: | XU zhen-hui YING Song-cheng SHEN Chao QU San-pu ZHENG Cong-yi |
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| Institution: | XU zhen-hui~1,YING Song-cheng~1,SHEN Chao~1,QU San-pu~1,ZHENG Cong-yi~ |
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| Abstract: | We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%. |
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| Keywords: | CSFV39-PK15 cell model Persistent infection Cell cycle Stability |
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