| 马铃薯Y病毒pl基因的克隆与序列分析 |
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| 引用本文： | 吴兴泉,陈士华,吴祖建,林奇英,谢联辉.马铃薯Y病毒pl基因的克隆与序列分析[J].Virologica Sinica,2003(4). |
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| 作者姓名： | 吴兴泉 陈士华 吴祖建 林奇英 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所，福建农林大学植物病毒研究所，福建农林大学植物病毒研究所，福建农林大学植物病毒研究所，福建农林大学植物病毒研究所 福建福州 350002，福建福州 350002，福建福州 350002，福建福州 350002，福建福州 350002 |
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| 基金项目： | 福建省农业厅和国家教育部资助项目(00183) |
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| 摘 要： | 利用依据马铃薯Y病毒(PVY)pl基因序列设计合成的一对引物YP1,YP2,以带毒烟草总RNA为模板,通过RT-PCR方法扩增得到了0.83kb的目的条带,测序结果表明为PVY pl基因。通过对PVY P1蛋白氨基酸序列分析发现PVY不同分离物间P1蛋白氨基酸序列存在明显差异,氨基酸序列同源性在64％～94％间。依据P1蛋白氨基酸序列建立了PVY系统关系树并对PVY进行了类型分析。
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| 关 键 词： | 马铃薯Y病毒 p1基因 序列分析 |
The Cloning And Sequence Analysis of The p1 Gene of Potato virus Y |
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| WU Xing-quan,CHEN Shi-hua,WU Zu-jian,LIN Qi-ying,XIE Lian-hui.The Cloning And Sequence Analysis of The p1 Gene of Potato virus Y[J].中国病毒学(英文版),2003(4). |
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| Authors: | WU Xing-quan CHEN Shi-hua WU Zu-jian LIN Qi-ying XIE Lian-hui |
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| Abstract: | With the specific primers YP1 and YP2 which were designed based on the reported Potato virus Y (PVY) p1 gene sequence, the target gene (0.83kb) was amplified by RT-PCR using the total RNA extracted from PVY infected tobacco plant as templates. The gene was cloned into plasmid and transformed then E. coli. DH5a sequenced. The result of sequence analysis shows it is the PVY p1 gene. The comparison shows that there are significant differences on the P1 amino acid sequence among the known PVY isolates, the identity ranges from 64% to 94%. Based on P1 amino acid sequence, the PVY phylogenic tree was established and the isolates of PVY were clustered into many branches. |
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| Keywords: | Potato virus Y pl gene Sequence analysis |
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