| 口蹄疫病毒感染BHK—21细胞的代谢热谱研究 |
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| 引用本文： | 刘欲文,张恒,顾潮江,郑从义,屈三甫,汪存信,常惠芸,吴继彬.口蹄疫病毒感染BHK—21细胞的代谢热谱研究[J].Virologica Sinica,2003,18(3):283-287. |
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| 作者姓名： | 刘欲文 张恒 顾潮江 郑从义 屈三甫 汪存信 常惠芸 吴继彬 |
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| 作者单位： | [1]武汉大学化学与分子科学学院，湖北武汉430072 [2]武汉大学生命科学学院，湖北武汉430072 [3]中国农科院兰州兽医研究所，甘肃兰州730046 |
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| 基金项目： | 国家重点基础研究发展规划项目“973”(G1999011904) |
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| 摘 要： | 本文应用高灵敏度的LKB2277生物活性检测仪测定FMDV感染BHK-21细胞的代谢热谱，并与传统方法测定的一步生长曲线进行比较，二者具有显著的相似性。结果表明，微量热法通过对细胞及其受病毒感染的细胞体系代谢热的测定，能有效地监测病毒在宿主细胞内增殖的过程。该方法还提供了一种动态连续分析病毒感染增殖的新手段。
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| 关 键 词： | 口蹄疫病毒 感染 BHK－21细胞 代谢热谱 一步生长曲线 |
Microcalorimetric Study of Foot-and-mouth disease virus Infecting BHK-21 Cell |
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| WU Ji-bin,LIU Yu-wen,Zhang Heng,GU Chao-jiang,ZHENG Cong-yi,QU San-fu,WANG Cun-xin,CHANG Hui-yun.Microcalorimetric Study of Foot-and-mouth disease virus Infecting BHK-21 Cell[J].中国病毒学(英文版),2003,18(3):283-287. |
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| Authors: | WU Ji-bin LIU Yu-wen Zhang Heng GU Chao-jiang ZHENG Cong-yi QU San-fu WANG Cun-xin CHANG Hui-yun |
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| Institution: | WU Ji-bin~1,LIU Yu-wen~2,Zhang Heng~2,GU Chao-jiang~1,ZHENG Cong-yi~1,QU San-fu~1,WANG Cun-xin~2,CHANG Hui-yun~3 |
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| Abstract: | Both microcalorimetric measurement analysis by using LKB2277 bioactivity monitor and traditional method used in one-step growth curve in virology were carded out on BHK-21 cell line infected by FMDV (Foot-and-Mouth Disease Virus). The results show that microcalorimetric study can be applied in virus infection research through measuring metabolic energy released from BHK-21 cell and BHK-21 cell infected by FMDV. Meantime this microcalorimetric method provided a kind of new dynamic continuous analysis method on the virus infection research. |
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| Keywords: | Foot-and-mouth disease virus(FMDV) BHK-21 Cell One step growth curve Microcalorimetric curve |
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