NDV CN株对几种人肿瘤细胞的杀伤作用及其机理的分析

本文研究了NDV-CN株对5株不同的人肿瘤细胞的体外杀伤作用。结果表明5株肿瘤细胞对NDV-CN敏感，于感染早期出现细胞收缩变圆，失去贴壁性，感染第5天时细胞存活率低于10％。其中尤以HEP3B细胞最敏感。但NDV-CN株对人二倍体细胞2BS有弱杀伤性。病毒感染早期可检测到感染细胞中有病毒核酸的复制、感染细胞表面有病毒蛋白的表达，脑浆内有病毒粒子存在。NDV-CN株主要诱导HEP3B及T24细胞产生凋亡，主要诱导Hep2、Hela及A549细胞产生坏死。

Analysis the Mechanism and the Oncolytic Effects of Newcastle disease virus to Some Strains of Tumor Cells

Mechanism of oncolysis of Newcastle disease virus (NDV) CN strain is studied in vitro in five carcinoma cells including HEP3B, T24, A549 and Hela cells, The pseudopods of the infected cells were retracted after 16h infected with 16HU/mL CN strain, the cells changed round and lost adhesion, the survival rates of the infected cells were lower than 10% by 120h. HEP3B cells was the most subjective to NDV. But CN strain seldom killed human normal cells 2BS. At early stage of infection (16h after infection) virus nucleic acid coding HN was detectable in cytoplasma by RT-PCR, NDV antigen was detectable by immunofluorescent test. At the same time, inspection of electric microscope showed NDV CN strain induced apoptosis in HEP3B and T24 cells. And induced necrosis in Hela, Hep2 and A549 cells.