| 芹菜夜蛾核型多角体病毒D克隆株某些理化性质的研究 |
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| 引用本文： | 高建明 张艳华 王艳 彭建新 洪华珠. 芹菜夜蛾核型多角体病毒D克隆株某些理化性质的研究[J]. Virologica Sinica, 2003, 18(2): 181-183 |
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| 作者姓名： | 高建明 张艳华 王艳 彭建新 洪华珠 |
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| 作者单位： | 华中师范大学昆虫所 湖北武汉430079(高建明，张艳华，王艳，彭建新)，华中师范大学昆虫所 湖北武汉430079(洪华珠) |
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| 基金项目： | 湖北省自然科学基金(2000 J 112) |
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| 摘 要： | DNA of Syngrapha falcifera nuclear polyhedrosis virus D-clone(Sfa-D clone) was extracted and digested by three kinds of restriction endonuclease,We calculated its molecular weight and measure its melting temperature,G C%, virus particle and polyhedrin were purified.The structural polypeptides and polyhedrin are analysed by SDS-PAGE.
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| 关 键 词： | 芹菜夜蛾核型多角体病毒 D克隆株 理化性质 DNA内切酶图谱 熔解温度 结构多肽 多角体蛋白 病毒杀虫剂 |
Some Character of Syngrapha falcifera nuclear polyhedrosis virus D-clone |
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| GAO Jian-ming,ZHANG Yan-hua,WANG Yan,PENG Jian-xin,HONG Hua-zhu. Some Character of Syngrapha falcifera nuclear polyhedrosis virus D-clone[J]. 中国病毒学(英文版), 2003, 18(2): 181-183 |
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| Authors: | GAO Jian-ming ZHANG Yan-hua WANG Yan PENG Jian-xin HONG Hua-zhu |
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| Affiliation: | GAO Jian-ming,ZHANG Yan-hua,WANG Yan,PENG Jian-xin**,HONG Hua-zhu |
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| Abstract: | DNA of Syngrapha falcifera nuclear polyhedrosis virus D-clone (Sfa-D clone) was extracted and digested by three kinds of restriction endonuclease. We calculated its molecular weight and measure its melting temperature, G+C%. virus particle and polyhedrin were purified. The structural polypeptides and polyhedrin are analysed by SDS-PAGE. |
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| Keywords: | Electrophegra of digestion fragments of DNA Melting temperature Structural polypeptids Polyhedrin |
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