| 猪繁殖与呼吸综合征与伪狂犬病混合感染的分子生物学快速诊断 |
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| 引用本文： | 许立华 王玲 苏鑫铭 王志亮 吴延功 陈溥言. 猪繁殖与呼吸综合征与伪狂犬病混合感染的分子生物学快速诊断[J]. Virologica Sinica, 2003, 18(6): 614-615 |
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| 作者姓名： | 许立华 王玲 苏鑫铭 王志亮 吴延功 陈溥言 |
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| 作者单位： | [1]南京农业大学农业部畜禽疫病诊断与免疫重点开放实验室，江苏南京，210095 [2]宁夏大学农学院动物科学系，宁夏银川，750105 [3]农业部动物检疫所，山东青岛，266032 |
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| 基金项目： | 国家“863”高技术发展计划资助项目(2001AA249012) |
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| 摘 要： | According to the nucleotide sequences of Porcine Reproductive and Respiratory Syndrome Virus VR2332 strain and Pseudorabies virus shanghai strain provided in GenBank, two pairs of primers were designed to amplify the N gene of PRRSV by RT-PCR and Tk gene of PRV by PCR. As a result, a rapid molecular diagnosis with high specificity and accuracy was set up.The result indicated that PRRSV was detected from 11 out of 33 samples, PRV was detected from 9 out of 33 samples, and co-infection by PRRSV and PRV was confirmed in 8 samples, the rate of co-infection was at 24.2%.
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| 关 键 词： | 猪繁殖与呼吸综合征 伪狂犬病 混合感染 快速诊断 |
Rapid Molecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome and Pseudorabies |
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| XU Li-hua. Rapid Molecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome and Pseudorabies[J]. 中国病毒学(英文版), 2003, 18(6): 614-615 |
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| Authors: | XU Li-hua |
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| Abstract: | According to the nucleotide sequences of Porcine Reproductive and Respiratory Syndrome Virus VR2332 strain and Pseudorabies virus shanghai strain provided in GenBank, two pairs of primers were designed to amplify the N gene of PRRSV by RT-PCR and Tk gene of PRV by PCR. As a result, a rapid molecular diagnosis with high specificity and accuracy was set up.The result indicated that PRRSV was detected from 11 out of 33 samples, PRV was detected from 9 out of 33 samples, and co-infection by PRRSV and PRV was confirmed in 8 samples, the rate of co-infection was at 24.2%. |
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| Keywords: | Porcine Reproductive and Respiratory Syndrome Pseudorabies Concurrent infection Rapid diagnosis |
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