| 猪圆环病毒2型及猪繁殖与呼吸综合症病毒的快速检测 |
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| 引用本文： | 芦银华,许立华,华修国,谈国蕾,黄伟坚,陈德胜,陈溥言.猪圆环病毒2型及猪繁殖与呼吸综合症病毒的快速检测[J].Virologica Sinica,2003,18(2):184-186. |
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| 作者姓名： | 芦银华 许立华 华修国 谈国蕾 黄伟坚 陈德胜 陈溥言 |
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| 作者单位： | [1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京，210095 [2]宁夏大学农学院动物科学系，宁夏银川，750105 [3]上海交通大学农学院，上海，200030 |
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| 基金项目： | 国家863高技术发展计划资助项目(2001AA249012)，上海市科技兴农攻关项目[农科攻字(2001)第3-5号] |
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| 摘 要： | According to the published genome sequences of Porcine circovirus type 2(PCV2)and Porcine reproductive and respiratory syndrome virus(PRRSV),primers were designed and PCR,RT-PCR were set up for the detection of PCV2 and PRRSV,respectively,With the established methods,38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2,27 samples were positive for PRRSV and among the above positive samples,18 samples were positive for both viruses,The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratiory diseases.
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| 关 键 词： | 猪圆环病毒2型 猪繁殖呼吸综合症病毒 共感染 检测 呼吸道疾病 PCR RT－PCR |
Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome virus |
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| LU Yin-hua,XU Li-hua,HUA Xiu-guo,TAN Guo-lei,HUANG Wei-jian,CHEN De-sheng,CHEN Pu-yan.Rapid Detection of Porcine circovirus Type 2 and Porcine reproductive and respiratory syndrome virus[J].中国病毒学(英文版),2003,18(2):184-186. |
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| Authors: | LU Yin-hua XU Li-hua HUA Xiu-guo TAN Guo-lei HUANG Wei-jian CHEN De-sheng CHEN Pu-yan |
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| Institution: | LU Yin-hua1,XU Li-hua1,2,HUA Xiu-guo3,TAN Guo-lei1,HUANG Wei-jian1,CHEN De-sheng1,CHEN Pu-yan1**, |
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| Abstract: | According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases. |
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| Keywords: | Porcine circovirus type 2 Porcine reproductive and respiratory syndrome virus Co-infection Detection |
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