Regeneration of transgenic plants expressing the GFP gene from rape cotyledonary and leaf explants: Effects of the genotype and ABA |
| |
Authors: | T G Hoang G N Raldugina |
| |
Institution: | 1.Russian State Agrarian University — Timiryazev Agricultural Academy,Moscow,Russia;2.Timiryazev Institute of Plant Physiology,Russian Academy of Sciences,Moscow,Russia |
| |
Abstract: | Cotyledons of five-day-old seedlings and leaves of 6-week-old plants of two rape cultivars (Brassica napus L., cvs. Westar and Podmoskovnyi) were co-cultured with the culture of Agrobacterium tumefaciens cells comprising the genetic construct with the marker gfp gene, on Murashige and Skoog nutrient medium supplemented with benzyladenine, NAA, and ABA in various combinations. A capacity
for regeneration on both types of explants was rather high, but leaf explants produced weakly differentiated shoots and most
of them were vitrificated. On cotyledonary explants of transformed rape plants of both cultivars expressing the gfp gene, regeneration frequency was 70%. On leaf explants, it was much lower (47% in cv. Westar and 28% in cv. Podmoskovnyi).
The gfp gene was expressed on all stages of shoot development. On primary, starting differentiation calli, we observed the strongest
fluorescence of GFP in meristematic and vascular tissues. On leaf blades, GFP fluorescence was much brighter in old than young
leaves; often it was observed only in the cell groups; it. PCR analysis of seed generation of transformants showed that some
plants did not follow the Mendelian inheritance of a monogenic trait (transgene) in self-pollinated plants. This phenomenon
could be explained as a result of meiotic recombination or production of genotypic chimeric organisms at regeneration. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|