Seroprevalence of antibodies against Anisakis simplex larvae among health-examined residents in three hospitals of southern parts of Korea |
| |
Authors: | Kim Jung Jo Jin Ok Choi Seon Hee Cho Min Kyoung Yu Hak Sun Cha Hee Jae Ock Meesun |
| |
Affiliation: | Department of Parasitology and Genetics, Kosin University College of Medicine, Busan 602-702, Korea. |
| |
Abstract: | The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis. |
| |
Keywords: | Anisakis simplex seroepidemiology excretory-secretory product crude extract ELISA western blotting |
本文献已被 PubMed 等数据库收录! |