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Sugar-attached upconversion lanthanide nanoparticles: A novel tool for high-throughput lectin assay |
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| Authors: | Yu Liu Takuya Kobayashi Masayuki Iizuka Tatsumi Tanaka Izumi Sotokawa Atsushi Shimoyama Yasutoshi Murayama Eigo Otsuji Shun-ichiro Ogura Hideya Yuasa |
| Affiliation: | 1. Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J2-10, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan;2. Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachihirokoji, Kamigyo-ku, Kyoto 602-8566, Japan;3. Frontier Research Center, Tokyo Institute of Technology, Japan |
| Abstract: | To create a novel high-throughput lectin assay (HTPLA) method based on the emission of a luminophore by highly penetrable near-infrared excitation, sugar-attached upconversion lanthanide nanoparticles (LNPs) were synthesized as a tool to highlight the aggregates caused by the sugar-mediated specific bridging between LNP and lectin. The emissions from a mannose-coated LNP in the aggregates with a mannose-binding lectin were much stronger than those from the non-aggregated samples, being sensitive enough for HTPLA. A galactose-coated LNP was also applicable to a macrophage aggregation assay for the sugar specificity of its surface lectin. |
| Keywords: | High-throughput lectin aggregation assay Lanthanides Luminescence Nanoparticles |
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