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Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility
Authors:Manuel Etzkorn  Thomas Raschle  Franz Hagn  Vladimir Gelev  Amanda J Rice  Thomas Walz  Gerhard Wagner
Institution:1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA;2. Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA;3. FB Reagents, 267 Pearl Street, Cambridge, MA 02139, USA
Abstract:
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  • Highlights? Bacteriorhodopsin (bR) is NMR accessible in nanodiscs and amphipols ? Nonconventional surfactants offer increased stability for cell-free expressed bR ? The bR-membrane interface is highly adaptable in the selected membrane mimetics ? Applications and limitations of the environments for NMR studies are discussed
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