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A novel pH sensitive water soluble fluorescent nanomicellar sensor for potential biomedical applications |
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| Authors: | Nikolai I Georgiev Rayna Bryaskova Rumiana Tzoneva Iva Ugrinova Christophe Detrembleur Stoyan Miloshev Abdullah M Asiri Abdullah H Qusti Vladimir B Bojinov |
| Institution: | 1. Department of Organic Synthesis, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Blvd., 1756 Sofia, Bulgaria;2. Department of Polymer Engineering, University of Chemical Technology and Metallurgy, 8 Kliment Ohridsky Str., 1756 Sofia, Bulgaria;3. Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Science, Acad. G. Bonchev Str., Bl.21, 1113 Sofia, Bulgaria;4. Institute of Molecular Biology, Bulgarian Academy of Science, Acad. G. Bonchev Str., Bl.21, 1113 Sofia, Bulgaria;5. Center for Education and Research on Macromolecules (CERM), Chemistry Department, University of Liege (ULg), Sart-Tilman B6a, 4000 Liège, Belgium;6. Chemistry Department, Faculty of Sciences, King Abdulaziz University, PO Box 80203, Jeddah 21589, Saudi Arabia;7. Center of Excellence for Advanced Materials Research (CEAMR), King Abdulaziz University, PO Box 80203, Jeddah 21589, Saudi Arabia |
| Abstract: | Herein we report on the synthesis and sensor activity of a novel pH sensitive probe designed as highly water-soluble fluorescent micelles by grafting of 1,8-naphthalimide–rhodamine bichromophoric FRET system (RNI) to the PMMA block of a well-defined amphiphilic diblock copolymer—poly(methyl methacrylate)–b-poly(methacrylic acid) (PMMA48–b-PMAA27). The RNI-PMMA48–b-PMAA27 adduct is capable of self-assembling into micelles with a hydrophobic PMMA core, containing the anchored fluorescent probe, and a hydrophilic shell composed of PMAA block. Novel fluorescent micelles are able to serve as a highly sensitive pH probe in water and to internalize successfully HeLa and HEK cells. Furthermore, they showed cell specificity and significantly higher photostability than that of a pure organic dye label such as BODIPY. The valuable properties of the newly prepared fluorescent micelles indicate the high potential of the probe for future biological and biomedical applications. |
| Keywords: | 1 8-Naphthalimide/rhodamine 6G bichromophore Fluorescent resonance energy transfer (FRET) pH sensor Well-defined PMMA–PMAA block copolymer Fluorescent micelles Living cells |
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