Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
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| Authors: | J M Pozuelo M M Lucas C de Lorenzo M Fernández-Pascual S Maldonado M R de Felipe |
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| Institution: | (1) Departamento de Fisiología y Bioquímica Vegetal, Centre de Ciencias Medioambientales, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain;(2) Departamento de Agroalimentación, Instituto Madrileño de Investigatión Agraria y Alimentaria, Comunidad de Madrid, Madrid;(3) Instituto Nacional de Tecnología Agropecuaria, Buenos Aires |
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| Abstract: | Summary Immunolocalization of alkaloids in lupin seeds (Lupinus spp.) has been performed by cryofixation and conventional methods. Alkaloids were localized in the protein bodies of the cotyledon cells. Some immunogold particles in the walls of these cells were also observed. There were no differences in the sites of localization between the two mentioned methods. X-ray microanalysis of elements showed the presence of P, Mg, S, and K in the protein bodies of cotyledon cells in lupin seeds. The role of K+ in alkaloids transport is discussed. |
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| Keywords: | Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies |
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