Ethanol fermentation by nystatin-resistant strains of Saccharomyces cerevisiae |
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Authors: | H. Grunwald-raij P. Margalith |
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Affiliation: | Department of Food Engineering and Biotechnology, Technion, Israel Institute of Technology, Technion City, Haifa, 32000, Israel |
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Abstract: | Nystatin-resistant mutants of haploid and polyploid strains of Saccharomyces cerevisiae were isolated by plating on gradient plates with increasing nystatin concentrations (60–3000 U/ml). Some of the mutants were defective in ergosterol biosynthesis, and produced zymosterol and cholestatetraenol-like sterols. Those mutants which do not form ergosterol produce less ethanol than the parent strains. They also had lower viability during fermentation of glucose solutions (8–13% vs. 33–47%). This became more pronounced in fermentations of higher concentrations of glucose. A nystatin-resistant but ergosterol-forming mutant had a similar fermentation capacity to the parent strain. |
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