Empirical validation of a two-state kinetic model for the B-Z transition of double-stranded poly[d(G-m5C)] |
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| Authors: | M A Fuertes José Manuel Pérez Víctor Manuel González Carlos Alonso |
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| Institution: | (1) Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM) Facultad de Ciencias, Universidad Autónoma de Madrid Cantoblanco, E-28049 Madrid, Spain e-mail: mafuertes@trasto.cbm.uam.es, Tel.: +34-91-3978463, Fax: +34-91-3974799, ES;(2) Departamento de Química Inorgánica, Facultad de Ciencias Universidad Autónoma de Madrid, Cantoblanco E-28049 Madrid, Spain, ES;(3) Department of Pathology, University of Michigan, Ann Arbor MI 48109-0668, USA, US |
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| Abstract: | 5 C)] in terms of the salt concentration, the absolute temperature, and the cooperativity index. The validity of the formula
was tested using circular dichroism spectroscopy after variation of the type of salt (NaCl, MgCl2), the salt concentration, and the temperature of the polynucleotide solution. A consequence of the proposed function is that
in conditions of high salt there is a predictable salt threshold which determines the particular molecular mechanism of the
B-Z transition. The paper also describes the way in which this threshold level is temperature dependent. A detailed comparison
of our data with the experimental data found by other authors is given. The function agrees quantitatively with the experiments
and explains the contrasting results found in the literature about the influence in the B-Z transition of both the temperature
and the polymer size.
Received: 21 April 1999 / Accepted: 9 September 1999 |
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| Keywords: | Circular dichroism spectroscopy Synthetic polynucleotide Salt dependence Temperature dependence Left-handed DNA |
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