Inhibition of Trichoderma reesei cellulase by sugars and solvents |
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Authors: | Holtzapple M Cognata M Shu Y Hendrickson C |
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Institution: | Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843. |
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Abstract: | Inhibition of Trichoderma reesei cellulase by sugars (glucose, delta-gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, delta-gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times. |
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