Western diet enhances hepatic inflammation in mice exposed to cecal ligation and puncture |
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Authors: | Chantal A Rivera LaTausha Gaskin Georg Singer Jeff Houghton Monique Allman |
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Institution: | (1) Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA, USA |
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Abstract: | Background Obese patients display an exaggerated morbidity during sepsis. Since consumption of a western-style diet (WD) is a major factor
for obesity in the United States, the purpose of the present study was to examine the influence of chronic WD consumption
on hepatic inflammation in mice made septic via cecal ligation and puncture (CLP). Feeding mice diets high in fat has been
shown to enhance evidence of TLR signaling and this pathway also mediates the hepatic response to invading bacteria. Therefore,
we hypothesized that the combined effects of sepsis and feeding WD on TRL-4 signaling would exacerbate hepatic inflammation.
Male C57BL/6 mice were fed purified control diet (CD) or WD that was enriched in butter fat (34.4% of calories) for 3 weeks
prior to CLP. Intravital microscopy was used to evaluate leukocyte adhesion in the hepatic microcirculation. To demonstrate
the direct effect of saturated fatty acid on hepatocytes, C3A human hepatocytes were cultured in medium containing 100 μM
palmitic acid (PA). Quantitative real-time PCR was used to assess mRNA expression of tumor necrosis factor-alpha (TNF-α, monocyte
chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), toll-like receptor-4 (TLR-4) and interleukin-8
(IL-8). |
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