Selection of streptavidin binders from a DNA-encoded chemical library |
| |
Authors: | Dumelin Christoph E Scheuermann Jörg Melkko Samu Neri Dario |
| |
Affiliation: | Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland. |
| |
Abstract: | DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequence serving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|