A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR |
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Authors: | Anne Purfield Amy Nelson Anita Laoboonchai Kanungnij Congpuong Phillip McDaniel R Scott Miller Kathy Welch Chansuda Wongsrichanalai Steven R Meshnick |
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Affiliation: | 1. Berlin Institute of Tropical Medicine, Berlin, Germany 2. Institute for International Health Sciences, Humboldt University, Berlin, Germany 3. School of Medicine and Health Sciences, University of Development Studies, Tamale, Ghana 4. Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany
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Abstract: | Background The erythrocyte binding antigen-175 (EBA-175) on Plasmodium falciparum merozoites mediates sialic acid dependent binding to glycophorin A on host erythrocytes and, therefore, plays a crucial role in cell invasion. Dimorphic allele segments have been found in its encoding gene with a 342 bp segment present in FCR-3 strains (F-segment) and a 423 bp segment in CAMP strains (C-segment). Possible associations of the dimorphism with severe malaria have been analysed in a case-control study in northern Ghana. Methods Blood samples of 289 children with severe malaria and 289 matched parasitaemic but asymptomatic controls were screened for eba- 175 F- and C-segments by nested polymerase chain reaction. Results In children with severe malaria, prevalences of F-, C- and mixed F-/C-segments were 70%, 19%, and 11%, respectively. The C-segment was found more frequently in severe malaria cases whereas mixed infections were more common in controls. Infection with strains harbouring the C-segment significantly increased the risk of fatal outcome. Conclusion The results show that the C-segment is associated with fatal outcome in children with severe malaria in northern Ghana, suggesting that it may contribute to the virulence of the parasite. |
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