Symbiosis-stimulated chitinase isoenzymes of soybean (Glycine mas (L.) Merr.) |
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| Authors: | Xie, Z Staehelin, C Wiemken, A Broughton, W Muller, J Boller, T |
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| Affiliation: | Botanisches Institut der Universitat Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland; Laboratoire de Biologie Moléculaire des Plantes Supérieures, Université de Genève, 1, Chemin de l'Impératrice, CH-1292 Chambesy/Geneve, Switzerland; Present address: Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, F-91998 Gif-sur-Yvette Cedex, France; Corresponding author e-mail: muellerjo@ubaclu.unibas.ch |
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| Abstract: | Isoforms of endochitinase in soybean were studied in relation to rootsymbiosis. Five selected cultivars differing in their nodulation potentialwere inoculated with two strains of Bradyrhizobiumjaponicum, the broad host-range Rhizobiumsp. NGR234, and with the mycorrhizal fungus Glomusmosseae. Total chitinase activity in nodules was up to 7-foldhigher than in uninoculated roots and in mycorrhizal roots. The chitinaseactivity in nodules varied depending on the strain-cultivar combination. Onsemi-native polyacrylamide gels, four acidic isoforms were identified. Twoisoforms (CH 2 and CH 4) were constitutively present in al analysedtissues. The other two isoforms (CH 1 and CH 3) were strongly induced innodules and were simulated in mycorrhizal roots as compared to uninoculatedroots. The induction of CH 1 varied in nodules depending on the soybeancultivar. This isoform was also stimulated in uninfected roots when theywere treated with tri-iodobenzoic acid, rhizobiallipochitooloigosaccharides (Nod factors) and chitotetraose. CH 3 was notaffected by these stimuli indicating that this isoform could represent amarker for enzymes induced in later stages of the symbioticinteractions.Key words: (Brady)rhizobium, chitinaseisoenzymes, mycorrhiza, (restricted) nodulation, Nod factors |
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