Growth of neonatal rat uterine luminal epithelium on extracellular matrix |
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| Authors: | William S. Branham Beverly D. Lyn-Cook Annette Andrews Maenola McDaniel Daniel M. Sheehan |
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| Affiliation: | (1) Division of Reproductive and Developmental Toxicology, Food and Drug Administration, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(2) Division of Comparative Toxicology, Food and Drug Administration, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(3) Pathology Associates, Inc., Food and Drug Administration, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(4) Department of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, 72079 Jefferson, Arkansas;(5) Departments of Biochemistry and Pharmacology, University of Arkansas for Medical Sciences, 72201 Little Rock, Arkansas |
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| Abstract: | Summary We have developed a tissue culture system using an extract of basement membrane (extracellular matrix) which promotes the in vitro growth and development of uterine luminal epithelium from the 5-day-old rat. Uterine luminal epithelium, free of stroma, was obtained as short tubes by trypsinization of uterine segments followed by mechanical separation. Epithelial segments were grown in a serum-free medium on culture dishes coated with an extracellular matrix. After 2 days, rapid cell growth resulted in monolayer cultures, which subsequently formed organoid structures similar to differentiated uterine glands present in uterine tissue taken from older rats. Electron microscopy of cultures revealed columnar cells with basally located nuclei, apical microvilli, lateral membranes with interdigitations, desmosomes, and secretory Golgi complexes, all features found in functioning uterine epithelium in vivo. This model will allow the in vitro investigation of the development of uterine epithelium-specific functions free of the influence of stromal cell factors. |
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| Keywords: | uterus epithelium culture extracellular matrix ultrastructure differentiation |
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