Overexpression of dimeric guanylyl cyclase cores of an atrial natriuretic peptide receptor. |
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Authors: | D S Thorpe S Niu E Morkin |
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Affiliation: | University Heart Center, University of Arizona College of Medicine, Tucson 85724. |
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Abstract: | Using a bacterial expression system, large amounts of the catalytic core of an atrial natriuretic peptide receptor guanylyl cyclase were produced and purified. After refolding the protein from a buffer containing urea, the enzyme had positively cooperative kinetics with a Hill coefficient, nH = 1.42 +/- 0.08. Size exclusion chromatography and denaturing polyacrylamide gel electrophoresis demonstrated that the enzyme is composed of homodimers with interacting catalytic sites. |
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