Directed evolution of ribosomal protein S1 for enhanced translational efficiency of high GC Rhodopseudomonas palustris DNA in Escherichia coli |
| |
Authors: | Bernstein Jeffrey R Bulter Thomas Shen Claire R Liao James C |
| |
Affiliation: | Department of Chemical and Biomolecular Engineering, UCLA, Los Angeles, California 90095, USA. |
| |
Abstract: | The expression of foreign DNA in Escherichia coli is important in biotechnological applications. However, the translation of genes from GC-rich organisms is inefficient in E. coli.To overcome this problem, we applied directed evolution to E. coli ribosomal protein S1. Two selected mutants enabled 12- and 8-fold higher expression levels from GC-rich DNA targets. General improvements in translation efficiency over a range of genes from Rhodopseudomonas palustris and E. coli was achieved using an S1 mutant selected against multiple genes from R. palustris. This method opens new opportunities for the expression of GC-rich genes in E. coli. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|