Rapid simple high-performance liquid chromatographic determination of paroxetine in human plasma |
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| Authors: | Jae-Gook Shin Kyung-Ah Kim Young-Ran Yoon In-June Cha Young-Hoon Kim Sang-Goo Shin |
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| Institution: | aDepartment of Pharmacology, Inje University College of Medicine and Clinical Pharmacology Center, Pusan Paik Hospital, Pusan 614-735, South Korea;bDepartment of Psychiatry, Inje University College of Medicine, Pusan Paik Hospital, Pusan 614-735, South Korea;cDepartment of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, South Korea |
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| Abstract: | A rapid, simple method for the measurement of paroxetine in human plasma by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection is described. This method includes only one-step extraction of paroxetine and dibucaine, an internal standard, with chloroform. Their recoveries were around 90%. The mobile phase, 10 mM phosphate buffer–acetonitrile (40:60, v/v) was eluted isocratically. Between- and within-day coefficients of variation were in the range of 1.9–9.4% and 2.3–13.3%, respectively. The detection limit was 0.2 ng/ml. The method we describe can be easily applied to the measurement of plasma paroxetine concentration for pharmacokinetic studies as well as for therapeutic drug monitoring in patients taking paroxetine. |
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| Keywords: | Paroxetine |
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