Pseudomonas oleovorans subsp. lubricantis subsp. nov., and Reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as Later Synonym of Pseudomonas oleovorans ATCC 8062T

Isolate RS1^T isolated from used metalworking fluid was found to be a Gram-negative, motile, and non-spore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1^T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C_18:1ω7c (32.6%), C_16:0 (25.5%), and C_15:0 ISO 2OH/C_16:1ω7c (14.4%). The sequence similarities of isolate RS1^T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062^T and 0.63 with P. pseudoalcaligenes ATCC17440^T. The DNA G + C content of isolate RS1^T was 62.2 mol.%. The DNA–DNA relatedness was 73.0% with P. oleovorans ATCC 8062^T and 79.1% with P. pseudoalcaligenes ATCC 17440^T. On the basis of morphological, biochemical, and molecular studies, isolate RS1^T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA–DNA relatedness (>70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440^T and P. oleovorans ATCC 8062^T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062^T = DSM 1045^T = NCIB 6576^T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1^T = ATCC BAA-1494^T = DSM 21016^T).