Antibody response to MfGL-II, a phosphocholine-containing major lipid of Mycoplasma fermentans membranes |
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Authors: | Gil Ben-Menachem Frauke Wagner Ulrich Zähringer Ernst Th Rietschel Shlomo Rottem |
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Institution: | Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006 Madrid, Spain;Institut für Mikrobiologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria |
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Abstract: | The choline-containing phosphoglycolipid, MfGL-II, is the major polar lipid of Mycoplasma fermentans PG18. Anti-MfGL-II antisera raised in rabbits using the purified MfGL-II as an immunogen were employed in immunogold electron microscopic and immunofluorescence studies showing that MfGL-II is uniformly distributed and exposed on the cell surface of M. fermentans cells. The specificity of the antibodies was determined by immunostaining of lipid extracts separated by thin layer chromatography. The antibodies recognize lipids specific to M. fermentans but did not cross-react with lipid extracts of M. penetrans, M. capricolum, M. gallisepticum or Acholeplasma laidlawii. As phosphocholine almost completely abolished antibody interaction with MfGL-II in an ELISA assay it is suggested that the anti-MfGL-II repertoire is composed primarily of anti-phosphocholine antibodies. The anti-MfGL-II antisera inhibit the attachment of M. fermentans to Molt-3 lymphocytes suggesting that MfGL-II plays a major role in M. fermentans-host cell interaction. |
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Keywords: | Rolling circle replication Strand-specific cleavage DNA polymerase I Runoff DNA synthesis assay |
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