Characterization of microsatellite DNA markers in the Emei moustache toads (<Emphasis Type="Italic">Leptobrachium boringii</Emphasis>) |
| |
Authors: | Ke Bi Duncan Deng M Kathrine A Crosby Jinzhong Fu |
| |
Institution: | (1) Department of Integrative Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada |
| |
Abstract: | We report ten microsatellite loci in the Emei moustache toads, Leptobrachium boringii. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Twenty-four individuals from one
breeding site were examined and ten loci were polymorphic. The number of alleles per locus ranged from 3–9 with an average
of 6.3/locus. The expected heterozygosity and observed heterozygosity ranged from 0.3874 to 0.8432, and from 0.4583 to 0.9167,
respectively. Cross-species amplification was tested in a closely related species L. leishanensis. These markers will be useful in future studies on characterizing the mating system of the species. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|