| Abstract: | Acetylcholinesterase (AChE) activity in the rat subfornical organ (SFO) was determined by the Koelle and Friedenwald method. Coronal sections showed a homogeneous reaction in the rostral region, which adopted a ring-like form in the anteromedial zone and a horseshoe pattern in the posteromedial zone. In the latter, AChE-specific positive neurons had been observed clearly in our sections. In sagittal sections the enzymatic reaction was intense in the anterior subependymal zone and dorsal region, and it continued caudally to the hippocampal area. The enzymatic activity was absent in the central region of the anteromedial zone and the subependymal region of the posteromedial zone. In sagittal sections the reaction was strongly positive in the periphery of the organ and negative in the posteromedial zone, near the choroid plexuses. Thus, we have shown that each region of the SFO presents a particular distribution of the AChE activity, related in some cases to neurons and in others to nerve fibers. |
