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Apical localization of 1-aminocyclopropane-1-carboxylic acid and its conversion to ethylene in etiolated pea seedlings |
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| Authors: | J E Taylor D G Grosskopf B A McGaw R Horgan I M Scott |
| Institution: | (1) Department of Botany and Microbiology, The University College of Wales, SY23 3DA Aberystwyth, Dyfed, UK;(2) Present address: Department of Physiology and Environmental Science, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD Loughborough, UK;(3) Present address: Rowett Research Institute, Greenburn Road, Bucksburn, AB2 9SB Aberdeen, UK |
| Abstract: | The biosynthetic basis for the high rates of ethylene production by the apical region of etiolated pea (Pisum sativum L.) seedlings was investigated. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) was quantified in extracts of various regions of seedlings by measuring isotopic dilution of a 2H-labelled internal standard using selected-ion-monitoring gas chromatography/mass spectrometry. The ACC levels in the apical hook and leaves were much higher than in the expanded internodes of the epicotyl. The capacity of excised tissue sections to convert exogenous ACC to ethylene was also much greater in the apical region, reflecting the distribution of soluble protein in the epicotyl.Abbreviations ACC 1-aminocyclopropane-1-carboxylic acid - FW fresh weight - GC/MS coupled gas chromatography/mass spectrometry - HPLC high-performance liquid chromatography |
| Keywords: | 1-Aminocyclopropane-1-carboxylic acid Ethylene biosynthesis Pisum (ethylene) |
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