Heterologous expression of cDNAs encoding monodehydroascorbate reductases from the moss, <Emphasis Type="Italic">Physcomitrella patens</Emphasis> and characterization of the expressed enzymes |
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Authors: | Damian P Drew Christina Lunde Jelle Lahnstein Geoffrey B Fincher |
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Institution: | (1) Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia;(2) Present address: Department of Plant Biology, Plant Biochemistry Laboratory, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, Frederiksberg C, 1871 Copenhagen, Denmark |
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Abstract: | Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) catalyses the reduction of the monodehydroascorbate (MDHA) radical to ascorbate,
using NADH or NADPH as an electron donor, and is believed to be involved in maintaining the reactive oxygen scavenging capability
of plant cells. This key enzyme in the ascorbate-glutathione cycle has been studied here in the moss Physcomitrella patens, which is tolerant to a range of abiotic stresses and is increasingly used as a model plant. In the present study, three
cDNAs encoding different MDHAR isoforms of 47 kDa were identified in P. patens, and found to exhibit enzymic characteristics similar to MDHARs in vascular plants despite low-sequence identity and a distant
evolutionary relationship between the species. The three cDNAs for the P. patens MDHAR enzymes were expressed in Escherichia coli and the active enzymes were purified and characterized. Each recombinant protein displayed an absorbance spectrum typical
of flavoenzymes and contained a single non-covalently bound FAD coenzyme molecule. The K
m and k
cat values for the heterologously expressed PpMDHAR enzymes ranged from 8 to 18 μM and 120–130 s−1, respectively, using NADH as the electron donor. The K
m values were at least an order of magnitude higher for NADPH. The K
m values for the MDHA radical were ∼0.5–1.0 μM for each of the purified enzymes, and further kinetic analyses indicated that
PpMDHARs follow a ‘ping–pong’ kinetic mechanism. In contrast to previously published data, site-directed mutagenesis indicated
that the conserved cysteine residue is not directly involved in the reduction of MDHA. |
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Keywords: | Ascorbate-glutathione cycle Enzyme kinetics Monodehydroascorbate reductase Physcomitrella Recombinant proteins Site-directed mutagenesis |
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