Interaction of the protein phosphatase 2A with the regulatory domain of the cystic fibrosis transmembrane conductance regulator channel |
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| Authors: | Vastiau Annick Cao Lishuang Jaspers Martine Owsianik Grzegorz Janssens Veerle Cuppens Harry Goris Jozef Nilius Bernd Cassiman Jean-Jacques |
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| Affiliation: | Department of Human Genetics, Division of Human Mutations and Polymorphisms, KULeuven, Herestraat 49, Postbus 602, 3000 Leuven, Belgium. |
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| Abstract: | A direct interaction of the regulatory domain (R domain) of the cystic fibrosis transmembrane conductance regulator protein (CFTR) with PR65, a regulatory subunit of the protein phosphatase 2A (PP2A), was shown in yeast two hybrid, pull-down and co-immunoprecipitation experiments. The R domain could be dephosphorylated by PP2A in vitro. Overexpression of the interacting domain of PR65 in Caco-2 cells, as well as treatment with okadaic acid, showed a prolonged deactivation of the chloride channel. Taken together our results show a direct and functional interaction between CFTR and PP2A. |
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| Keywords: | AA, amino acids CFTR, cystic fibrosis transmembrane conductance regulator protein PKA, protein kinase A PKC, protein kinase C OA, okadaic acid PP2A, protein phosphatase 2A PR1-10, first 10 HEAT repeats of PR65 PR65, 65 kDa regulatory subunit of PP2A R domain, regulatory domain of CFTR Y2H, yeast two hybrid |
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