| 人红细胞膜上阴离子交换蛋白和葡萄糖运输蛋白的相互影响 |
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| 引用本文： | 张志鸿,彭锋,李晓泉,周汉清,胡小健. 人红细胞膜上阴离子交换蛋白和葡萄糖运输蛋白的相互影响[J]. Acta biochimica et biophysica Sinica, 1996, 0(6) |
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| 作者姓名： | 张志鸿 彭锋 李晓泉 周汉清 胡小健 |
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| 作者单位： | 复旦大学生理学和生物物理学系 |
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| 摘 要： | 用细胞松弛素Ｂ抑制红细胞膜上葡萄糖运输蛋白（ＧｌｕＴ－１）对葡萄糖的运输，观察到对阴离子运输有促进作用。当ＧｌｕＴ－１结合其底物分子－葡萄糖后同样加快了阴离子运输速率．另一方面，实验办给出了葡萄糖跨膜运输特性和Ｃｌ－离子浓度的关系，表明随着Ｃｌ－离子浓度的加大能使葡萄糖运输过程中Ｋｍ减小、Ｖ（ｍａｘ）增大。这些结果表明了在完整红细胞膜上阴离子交换蛋白Ｂａｎｄ３和ＧｌｕＴ－１之间存在着双向联系，即一种膜蛋白的构象改变能影响另一种膜蛋白的功能。
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| 关 键 词： | 红细胞；阴离子交换蛋白；葡萄糖运输蛋白；细胞松弛素B；膜蛋白相互作用 |
Interaction Between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes |
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| ZHANG Zhi-Hong,PENG Feng,LI Xiao-Quan,ZHOU Han-Qing and HU Xiao-Jian. Interaction Between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes[J]. Acta biochimica et biophysica Sinica, 1996, 0(6) |
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| Authors: | ZHANG Zhi-Hong PENG Feng LI Xiao-Quan ZHOU Han-Qing HU Xiao-Jian |
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| Abstract: | Cytochalasin B was used to inhibit the glucose iransport through the glucose transport protein(GluT-1)in red cell membranes.It was observed that the anion transport was increased at the same time.The rate of anion transport was also inereased when GluT-1 was bound with its substrate molecule-glucose.On the other hand,the experimental result on the relationship between glucose transport and Cl-concentration showed that Km was decreased and Vmax increased following the increase of Cl-concentration. There would be a bidirectional linkage between Band 3(anion exchange protein)and GluT-1 in the intact red cell membranes.In other words,the conformation change of membrane protein could affect the function of other kinds of membrane proteins. |
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| Keywords: | Red blood cells Anion exchange Protein Glucose transport protein Cytochalasin B Interaction between membrane proteina |
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