| 叶绿体小分子RNA的分离纯化和鉴定 |
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| 引用本文： | 程振起,张虹,谷丽雅,赵晜,曹维国. 叶绿体小分子RNA的分离纯化和鉴定[J]. Acta Botanica Sinica, 1985, 0(2) |
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| 作者姓名： | 程振起 张虹 谷丽雅 赵晜 曹维国 |
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| 作者单位： | 中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所，中国科学院生物物理所 武汉大学生物系，武汉大学生物系，武汉大学生物系 |
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| 摘 要： | 本文采用低速匀浆、过筛的方法从植物叶中分离得到了完整、纯净的叶绿体。将叶绿体与提取缓冲液、苯酚混合匀浆抽提叶绿体 RNA。通过聚丙烯酰胺凝胶电泳与文献已发表的已知酵母5S RNA、菠菜叶绿体4.5S RNA 及小麦5S RNA、4.5S RNA 的电泳迁移距离进行比较,发现芹菜叶绿体中小分子 RNA(沉降系数为5S 左右的 RNA)中除含有5S RNA 和4S RNA 外,还含有两种4.5S RNA。而水杉和银杏叶绿体小分子 RNA 中只含有5S RNA 和4S RNA。
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| 关 键 词： | 叶绿体小分手 RNA 纯化 鉴定 |
PURIFICATION AND CHARACTERIZATION OF LOW MOLECULAR WEIGHT RNA OF CHLOROPLASTS |
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| Cheng Zhen-qi Zhang Hung Gu Li-ya Zhao Kun Cao Wei-guo. PURIFICATION AND CHARACTERIZATION OF LOW MOLECULAR WEIGHT RNA OF CHLOROPLASTS[J]. , 1985, 0(2) |
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| Authors: | Cheng Zhen-qi Zhang Hung Gu Li-ya Zhao Kun Cao Wei-guo |
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| Abstract: | Purified intact chloroplasts were abtained from plant leaves by homo- genizing at low speed and filtration.The chloroplast RNA were extracted from the chloroplasts with a mixture of extracting buffer and phenol.The chloroplasts of Oenan- the stolonifera DC.contain not only 5S RNA and 4S RNA but also 4.5S RNA among low molecular weight RNA.However there are only 5S RNA and 4S RNA in chloroplasts of Metasequoia qlyptostroides and Ginkgo biloba leaves. |
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| Keywords: | Chloroplast Chloroplast RNA Low molecular RNA Purification |
| 本文献已被 CNKI 等数据库收录！ |
