| ELISA方法测定血清中Hib多糖抗体含量的条件比较研究 |
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| 引用本文： | 李红,唐静,李茂光,李亚南,何莉,叶强. ELISA方法测定血清中Hib多糖抗体含量的条件比较研究[J]. 微生物学免疫学进展, 2009, 37(1) |
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| 作者姓名： | 李红 唐静 李茂光 李亚南 何莉 叶强 |
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| 作者单位： | 中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 分别以牛血清白蛋白和人血清白蛋白作为封闭液的主要成分测定7份血清中抗b型流感嗜血杆菌荚膜多糖的抗体含量,结果显示,两组数据之间没有显著性差异(P>0.05)。分别用HRP标记的羊抗人IgG和HRP标记的鼠抗人IgG测定该7份血清,结果显示两组结果无显著性差异(P>0.05)。同时,将血清反应的温度和时间由37℃1.5h改为4℃16h(过夜),结果显示两者无显著性差异(P>0.05)。从而优化了该方法。
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| 关 键 词： | b型流感嗜血杆菌 酶联免疫测定 抗体 |
Comparative study of test conditions of ELISA for measuring the amount of antibody against Haemophilus influenzae type b polysaccharide |
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| Abstract: | Bovine serum albumin and human serum albumin were used as blocking reagent respectively to measure the amount of IgG against capsular polysaccharide(CPS)of Haemophilus influenzae type b(Hib) in seven human serum samples.Two kinds of HRP-labelled antibody were used respectively to assay IgG against CPS of Hib contained in the same seven samples.The incubate tempreture and time were also changed from 37℃ for 1.5h to 4℃ for 16h(over night).The results showed that there is no significant statistical difference between the two groups which were compared(P>0.05).Thus the comparative study can give more choices in the assay of measuring the level of Hib antibody. |
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| Keywords: | Haemophilus influenzae type b ELISA Antibody |
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