Stereochemistry of NADPH oxidation by dihydropyrimidine dehydrogenase from pig liver. |
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| Authors: | B Podschun |
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| Institution: | Theodor-Boveri-Institut fuer Biowissenschaften (Biozentrum), Universitaet Wuerzburg, Germany. |
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| Abstract: | Dihydropyrimidine dehydrogenase reduces uracil to 5,6-dihydrouracil in a strictly NADPH-dependent reaction. Either by analysing the 1H-NMR spectra of the NADP+ products formed or by determination of the kinetic isotope effects of stereospecifically deuterated coenzymes dihydropyrimidine dehydrogenase was found to abstract specifically the pro-S hydrogen of NADPH, making it a member of the B-side stereospecific class of dehydrogenases. |
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