Variations in myocardial CPK and Na+-K+ ATPase following normo- and hypothermic exposure to dimethyl sulfoxide and glycerol. |
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Authors: | R F Cox J G Baust |
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Affiliation: | Program in Physiology, Department of Biology, University of Houston, Houston, Texas 77004 U.S.A. |
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Abstract: | Exposure of canine myocardial tissue homogenates to Me2SO glycerol (20 to 60%) for periods up to 8 hr resulted in significant alterations in enzyme activity at 0 °, 18 °, and 37 °C. Both CPK and Na+-K+ ATPase demonstrate anomalous enhancement of activity at each temperature with glycerol. Me2SO provides a similar enhancement of Na+-K+ ATPase activity at hypothermic temperatures up to 40%. Thereafter, nearly complete inhibition resulted. Under normothermic conditions complete Me2SO inhibition occurred at 40 °. CPK activity diminished in a linear fashion after 4 hr at 18 ° and 37 ° but was unaffected by up to 40% Me2SO at 0 °C. The results suggest that disruption of the CPK-Na+-K+ ATPase systems may be minimized by hypothermic perfusion at low cryoprotectant concentrations. |
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Keywords: | To whom reprint requests should be addressed. |
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