Pressurized water extraction of β‐glucan enriched fractions with bile acids‐binding capacities obtained from edible mushrooms |
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Authors: | Marimuthu Palanisamy Laila Aldars‐García Alicia Gil‐Ramírez Alejandro Ruiz‐Rodríguez Francisco R Marín Guillermo Reglero Cristina Soler‐Rivas |
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Institution: | Dept. of Production and Characterization of Novel Foods, CIAL–Research Inst. in Food Science (UAM+CSIC), Madrid, Spain |
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Abstract: | A pressurized water extraction (PWE) method was developed in order to extract β‐glucans with bile acids‐binding capacities from cultivated mushrooms (Agaricus bisporus, Lentinula edodes, and Pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. Extraction yields were higher in individual than sequential extractions being the optimal extraction parameters: 200°C, 5 cycles of 5 min each at 10.3 MPa. The crude polysaccharide (PSC) fractions, isolated from the PWE extracts contained mainly β‐glucans (including chitooligosaccharides deriving from chitin hydrolysis), α‐glucans, and other PSCs (hetero‐/proteo‐glucans) depending on the extraction temperature and mushroom strain considered. The observed bile acids‐binding capacities of some extracts were similar to a β‐glucan enriched fraction obtained from cereals. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:391–400, 2014 |
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Keywords: | accelerated solvent extraction β ‐glucans bile acids oyster mushroom shiitake mushroom white button mushroom cholesterol |
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