A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells |
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| Authors: | Fulya Ekiz Kanik Didem Ag Muharrem Seleci Firat Baris Barlas Melis Kesik Gonul Hizalan Hava Akpinar Suna Timur Levent Toppare |
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| Affiliation: | 1. Dept. of Biotechnology, Middle East Technical University, Ankara, Turkey;2. Dept. of Biochemistry, Faculty of Science, Ege University, Bornova‐Izmir, Turkey;3. Dept. of Chemistry, Middle East Technical University, Ankara, Turkey;4. Dept. of Polymer Science and Technology, Middle East Technical University, Ankara, Turkey;5. Ege University, Inst. on Drug Abuse, Toxicology and Pharmaceutical Science (BATI), Bornova, Izmir, Turkey;6. The Center for Solar Energy Research and Application (GUNAM), Middle East Technical University, Ankara, Turkey |
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| Abstract: | We describe a modification and post‐functionalization technique for a donor–acceptor–donor type monomer; 6‐(4,7‐bis(2,3‐dihydrothieno[3,4‐b][1,4]dioxin‐5‐yl)‐2H‐benzo[d][1,2, 3]triazol‐2‐yl)hexan‐1‐amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:952–959, 2014 |
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| Keywords: | fluorescence microscopy cell imaging folic acid conjugation |
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