Chemical Constituents and Activities of the Essential Oil from Myristica fragrans against Cigarette Beetle Lasioderma serricorne |
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Authors: | Shu‐Shan Du Kai Yang Cheng‐Fang Wang Chun‐Xue You Zhu‐Feng Geng Shan‐Shan Guo Zhi‐Wei Deng Zhi‐Long Liu |
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Affiliation: | 1. State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, P.?R. China (phone: +86‐10‐62208022);2. Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, P.?R. China;3. Analytical and Testing Center, Beijing Normal University, Haidian District, Beijing 100875, P.?R. China;4. Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, P.?R. China |
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Abstract: | Essential oil extracted from nutmeg seeds (Myristica fragrans Houtt .) by hydrodistillation was subjected to GC/MS and GC analysis. A total of 27 constituents were identified, of which eugenol (19.9%), methylisoeugenol (16.8%), methyleugenol (16.7%), sabinene (11.8%), and terpinen‐4‐ol (8.5%) were the major components. The essential oil was tested against Lasioderma serricorne for insecticidal and repellent activity, the LD50 value at the end of 24 h exposure period was 19.3 μg/adult. Six active compounds were isolated by bioassay‐guided fractionation. They were identified as eugenol ( 1 ), methyleugenol ( 2 ), methylisoeugenol ( 3 ), elemicin ( 4 ), myristicin ( 5 ), and safrole ( 6 ). Among these isolates, 4 showed the strongest contact toxicity against L. serricorne adults with an LD50 value of 9.8 μg/adult. Repellency of crude oil and active compounds were also determined. Compounds 1, 2, 4 , and 5 were strongly repellent against the cigarette beetle and exhibited the same level of repellency compared with the positive control, DEET. The results indicate that the essential oil of M. fragrans and its active constituents have potential for development as natural insecticides and repellents to control L. serricorne. |
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Keywords: | Myristica fragrans Lasioderma serricorne Essential oils Contact toxicity Repellent activity |
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