A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe |
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Authors: | S Huck G Keyeux N Ghanem M P Lefranc G Lefranc |
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Institution: | Laboratoire d''Immunogénétique Université des Sciences et Techniques du Languedoc UA CNRS1191, 34060 Montpellier Cédex, France |
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Abstract: | We report the first specific human immunoglobulin subclass probe which was obtained by subcloning the gamma 3 hinge region. This specific gamma 3 probe allowed us to identify with certainty the C gamma 3 gene on Southern genomic blots, to describe the first C gamma 3 restriction fragment length polymorphism (EZZ gamma 3 RF) and to show that an IgG3 selective deficiency, previously described serologically, was not due to a deletion of the C gamma 3 gene. Such a probe should be particularly useful for screening libraries from individuals with IgG3 immunodeficiencies or presenting unusual C gamma 3 genes and, consequently, for studying the C gamma gene evolution. |
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