| 二氧化硅纳米材料固定中性脂肪酶的条件优化及其特性 |
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| 引用本文： | 金杰,杨艳红,吴克,王华林,刘斌,俞志敏.二氧化硅纳米材料固定中性脂肪酶的条件优化及其特性[J].生物工程学报,2009,25(12):2003-2007. |
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| 作者姓名： | 金杰 杨艳红 吴克 王华林 刘斌 俞志敏 |
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| 作者单位： | 1. 合肥学院应用酶学与工程重点实验室城市固废处理与资源化利用安徽省工程技术研究中心,合肥,230022
2. 合肥工业大学化学工程学院,合肥,230009 |
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| 基金项目： | 安徽省科技厅重点攻关项目(No.07010202077)资助~~ |
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| 摘 要： | 以二氧化硅纳米材料为载体,采用吸附法对脂肪酶进行固定化,研究了不同条件对固定化脂肪酶的催化活性的影响,得到最佳的固定化条件:给酶量为28300U/g,固定化温度为45oC,pH值为7.5,时间为10h,此时固定化酶的活力约为3867U/g载体。固定化酶的最适反应温度为45oC,比游离酶的反应温度高5oC,最适pH下降到5.5,低于游离酶的反应pH(pH7)。固定化酶的热稳定性和pH稳定性较游离酶有了很大的提高,其在70oC以下能保持70%以上的酶活力,而游离酶在50oC下残余酶活力仅为30%。在pH5～8的范围内,固定化酶的酶活力能保持50%以上,而游离酶只能保持20%左右。用固定化的中性脂肪酶催化不同的油品,即大豆油、菜籽油及泔水油生产生物柴油,菜籽油的酯化率最高。
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| 关 键 词： | 二氧化硅纳米材料 固定化 脂肪酶 生物柴油 |
| 收稿时间： | 2009/10/9 0:00:00 |
Optimize conditions and activities for neutrophil lipase immobilized by nano-silica dioxide |
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| Jie Jin,Yanhong Yang,Ke Wu,Hualin Wang,Bin Liu and Zhimin Yu.Optimize conditions and activities for neutrophil lipase immobilized by nano-silica dioxide[J].Chinese Journal of Biotechnology,2009,25(12):2003-2007. |
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| Authors: | Jie Jin Yanhong Yang Ke Wu Hualin Wang Bin Liu and Zhimin Yu |
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| Institution: | Key Laboratory of Applied Enzymology & Engineering, the Engineering Technology Research Center for Municipal Solid Waste and Resource Application of Anhui Province, Hefei University, Hefei 230022, China;School of Chemical Engineering, Hefei University of Technology, Hefei 230039, China;Key Laboratory of Applied Enzymology & Engineering, the Engineering Technology Research Center for Municipal Solid Waste and Resource Application of Anhui Province, Hefei University, Hefei 230022, China;School of Chemical Engineering, Hefei University of Technology, Hefei 230039, China;Key Laboratory of Applied Enzymology & Engineering, the Engineering Technology Research Center for Municipal Solid Waste and Resource Application of Anhui Province, Hefei University, Hefei 230022, China;Key Laboratory of Applied Enzymology & Engineering, the Engineering Technology Research Center for Municipal Solid Waste and Resource Application of Anhui Province, Hefei University, Hefei 230022, China |
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| Abstract: | We used adsorption method to immobilize the lipase by using nano-silica dioxide as the carrier.And we also studied different immobilization conditions effects on the catalytic activity.We got optimize immobilization conditions which were as follow the lipase quantity of 28 300 U/g,temperature of 45oC,pH of 7.5 and treated for 10 h.Under these conditions the immobilized lipase activity yield 3867 U/g carrier.The best reactive temperature for immobilized lipase was 45oC and higher than 5oC for free enzyme,and... |
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| Keywords: | nano-silica dioxide immobilization lipase biodiesel |
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