Derivation of human embryonic stem cells from single blastomeres |
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Authors: | Klimanskaya Irina Chung Young Becker Sandy Lu Shi-Jiang Lanza Robert |
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Affiliation: | Advanced Cell Technology, Worcester, Massachusetts 01605, USA. |
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Abstract: | This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3-4 months. |
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