Intracellular pH regulation in rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) hepatocytes: the activity of sodium/proton exchange is oxygen-dependent |
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Authors: | A?Tuominen E?Rissanen A?Bogdanova Email author" target="_blank">M?NikinmaaEmail author |
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Institution: | Department of Biology, University of Turku, 20014 Turku, Finland. |
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Abstract: | We studied pH regulation in freshly isolated rainbow trout hepatocytes using microspectrofluorometry with the fluorescent dye BCECF. In accordance with earlier data on rainbow trout hepatocytes, ion substitution (N-methyl D-glucamine for sodium and gluconate for chloride) and transport inhibitor 10 microM M methyl isobutyl amiloride (MIA) to inhibit sodium/proton exchange and 100 microM DIDS to inhibit bicarbonate transport] studies in either Hepes-buffered or bicarbonate/carbon dioxide-buffered media (extracellular pH 7.6) indicated a role for sodium/proton exchange, sodium-dependent bicarbonate transport, and sodium-independent anion exchange in the regulation of hepatocyte pH. In Hepes-buffered medium, the activity of the sodium/proton exchanger (i.e. proton extrusion inhibited by MIA) was greater at 1% than at 21% oxygen. The oxygen dependency of the sodium/proton exchange is not caused by hydroxyl radicals, which appear to mediate the oxygen sensitivity of potassium-chloride cotransport in erythrocytes. |
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