消化道癌原代培养体外药敏试验的研究

目的 研究肠癌、胃癌、肝癌细胞在8种不同抗癌药物作用下的细胞抑制率,以筛选出敏感的化疗药物.方法 采用四甲基偶氮唑蓝着色法（MTT）,对97例消化道癌（43例肠癌、33例胃癌、21例肝癌）新鲜瘤组织进行原代细胞培养,同时进行顺铂（DDP）、丝裂霉素（MMC）、卡铂（CBP）、5-氟尿嘧啶（5-FU）、表阿霉素（EADM）、长春新碱（VCR）、羟基喜树碱（OPT）和环磷酰胺（CTX）8种常用化疗药物敏感检测,并对其结果作比较.结果 在肠癌中高度敏感率大于50%药物依次为MMC、5-FU、DDP、CBP.总敏感率大于50%的药物依次为DDP、MMC、5-FU、CBP.在胃癌中高度敏感率大于50%的药物依次为DDP、CBP.总敏感率大于50%的药物依次为DDP、CBP、MMC、5-FU、CTX、VCR.其中胃癌对CTX、VCR的敏感率明显高于肠癌细胞（P＜0.05）.肝癌细胞中高度敏感率＞50%的药物为零,而总敏感率＞50%的药物依次排序为MMC、EADM、CBP.结论 消化道肿瘤细胞对化疗药物的选择虽有一定共性,但同种肿瘤的不同个体对同种化疗药物的敏感性差异却存有显著性.体外检测消化道肿瘤对化疗药物的敏感性可为临床化疗提供指导.

The inhibitory effect of chemotherapy drug on digestive tract carcinomas

Objective To explore the effective chemotherapy drug to digestive tract carcinomas by contrasting their sensitivity to different chemotherapy drugs.Methods Fresh tissue from 97 cases of digestive tract carcinomas(colorectal carcinomas 43 cases,gastric carcinomas 33 cases,liver cancer 8 cases) were taken to test their sensitivity to chemotherapy drugs DDP,MMC,CBP,5-FU,EADM,VCR,OPT and CTX by the method of MTT.The effects were compared among different carcinomas to these chemotherapy drugs.Results Colorectal carcinomas were sensitive to MMC,5-FU,DDP and CBP,and all the inhibitory rates were more than 50%.Gastric carcinomas were sensitive to DDP,CBP,MMC,5-FU,CTX and VCR,and the inhibitory rates were more than 50% of CTX and VCR.Liver carcinomas were sensitive to MMC,EADM and CBP,and none of the inhibitory rates were more than 50%.Conclusions Although digestive tract carcinomas have common characteristic of being sensitive or resistant to some chemotherapy drugs,there exist different inhibitory rates among different individuals.Therefore it is very important to carry out the sensitivity test to chemotherapy drug individually in vitro.