Endocytosis of α1-acid glycoprotein variants by human monocytic lineage cells |
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Authors: | Valrie Carpentier Patrick Midoux Michel Monsigny Annie-Claude Roche |
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Institution: | Valérie Carpentier,Patrick Midoux,Michel Monsigny,Annie-Claude Roche |
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Abstract: | Summary— Human α1-acid glycoprotein (AGP or orosomucoid) is a major glycoprotein of plasma. AGP can be separated on immobilized concanavalin A into three variants bearing none (AGP A), one (AGP B) or two (AGP C) biantennary glycans. In this paper, we show, using flow cytometry and confocal microscopy, that AGP C which is eluted from concanavalin A with mannose, binds to human monocytes, monocyte-derived macrophages as well as human promonocytic cell lines such as THP1 or U937. Conversely HL60, a promyelocytic cell line, does not express the surface AGP C binding protein. AGP C is internalized and degraded with an efficiency depending on the state of differentiation of these cells. In contrast, AGP A which is not recognized by concanavalin A, does not bind to any of these cells. |
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Keywords: | α 1-acid glycoprotein endocytosis flow cytometry monocytic cells |
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