大豆异黄酮对骨质疏松大鼠模型的作用探讨

目的 研究大豆异黄酮促进骨质疏松大鼠骨形成的作用及其肠道微生态的变化。 方法 从60只SPF级SD雌性大鼠中随机挑选50只建立去卵巢骨质疏松大鼠模型,余下10只为假手术组。将建模成功的大鼠随机分为5组,模型组、大豆异黄酮高、中、低剂量组(320 mg/kg、160 mg/kg、80 mg/kg的大豆异黄酮)、阿仑膦酸钠组(1 mg/kg阿仑膦酸钠),每日1次,治疗10周。比较治疗前后大鼠骨密度、血清1型前胶原N端前肽(P1NP)、骨碱性磷酸酶(BALP)、血清骨钙素(BGP)水平和肠道微生物变化。 结果 治疗后,模型组大鼠骨密度、血清P1NP、BALP、BGP水平均低于假手术组(P结论 大豆异黄酮可提高骨密度,提高血清骨形成指标水平,促进骨质疏松大鼠骨形成,还可改善大鼠肠道菌群。

Effect of soybean isoflavones on osteoporosis rat models

Objective To observe the effect of soybean isoflavones(SIs) on bone formation and the changes of intestinal microecology in osteoporosis rats. Methods Fifty out of sixty SPF grade SD female rats were randomly selected to establish the models of ovariectomized osteoporosis, with the remaining 10 as the sham operation group(1 mL/100 g saline).After 3 months the osteoporosis models were successfully established, they were randomly divided into the model group(saline 1 mL/100 g), SIs high dose(SIs 320 mg/kg) group, SIs medium dose(SIs 160 mg/kg) group, SIs low dose(SIs 80 mg/kg) group or alendronate sodium(AS)(1 mg/kg) group. The changes in rat bone mineral density, serum type 1 procollagen Nterminal propeptide(P1NP), bone alkaline phosphatase(BALP), serum osteocalcin(BGP) and intestinal microbe before and after treatment were compared among groups. Results After treatment, the bone mineral density and serum levels of P1NP, BALP, and BGP in the model group were lower than those in the sham operation group(PConclusion Soybean isoflavones can increase bone density and serum bone formation indexes, promote bone formation in rats with osteoporosis, reduce the pathological changes of rat femur tissues, and improve intestinal microecology of rats by increasing the growth of beneficial bacteria while inhibiting the growth of pathogenic bacteria.