乳杆菌发酵上清对耐药白假丝酵母生物膜的作用

目的 研究德氏乳杆菌(Lactobacillus delbrueckii,L.delbrueckii)和发酵乳杆菌(Lactobacillus fermentum,L.fermentum)发酵上清液对3株耐氟康唑白假丝酵母临床分离株CA3、CA6、CA8生物膜形成和分散的作用。 方法 通过MIC试验,确认3株白假丝酵母临床分离株对氟康唑耐药；用96孔板构建体外白假丝酵母生物膜；用棋盘法分别检测L.delbrueckii、L.fermentum发酵上清液与氟康唑联用对3株白假丝酵母的作用；用XTT法对生物膜形成量进行定量分析；检测L.delbrueckii、L.fermentum发酵上清液与氟康唑联用对白假丝酵母时间-生长曲线的影响；显微镜拍照检测L.delbrueckii、L.fermentum发酵上清液单独和氟康唑联用对白假丝酵母生物膜形态的影响；平板培养法检测L.fermentum发酵上清液对白假丝酵母黏附作用的影响。 结果 CA3、CA6、CA8三株临床分离菌株对氟康唑耐药,MIC浓度均为8 μg/mL。L.delbrueckii与L.fermentum发酵上清液与氟康唑联用对3株耐药型白假丝酵母的生物膜形成与分散均未表现出协同作用,且发酵上清液与氟康唑联用效果不如发酵上清液单独处理效果好,L.fermentum发酵上清液对生物膜的分散作用较L.delbrueckii发酵上清液强。L.fermentum发酵上清液抑制白假丝酵母的初始黏附。 结论 L.delbrueckii、L.fermentum发酵上清液单独应用时均对耐氟康唑白假丝酵母生物膜的形成与分散有干预作用,与氟康唑联用时未表现出协同作用,抑制耐药白假丝酵母生物膜的作用可能与抑制菌丝形成和起始黏附有关。

The effect of Lactobacillus fermentation supernatant on drugresistant Candida albicans biofilm

Objective To observe the effect of Lactobacillus delbrueckii fermented supernatant and Lactobacillus fermentum fermented supernatant on biofilm formation and dispersion of Fluconazole resistant Candida albicans obtained clinically. Methods Through the MIC experiment, three clinical isolates (CA3, CA6 and CA8) were identified as resistant to Fluconazole. The experiment methods include constructing C.albicans biofilm with 96well plates; detecting the effects of fermented supernatants by L.delbrueckii and L.fermentum as well as Fluconazole on the three strains with chessboard method; quantitatively analyzing the amount of biofilm by XTT method; observing the effects of L.delbrueckii and L.fermentum supernatants combined with Fluconazole on the timegrowth curve of C.albicans; using microscope photographs to detect the effects of L.delbrueckii and L.fermentum fermentation supernatants alone and combined with Fluconazole on the morphology of C.albicans biofilm; using plate culture method to detect the effects of L.fermentum fermentation supernatant on the adhesion of C.albicans. Results The three clinical isolates were confirmed as drugresistant by MIC test and the MIC was 8 μg/mL. The combination of L.delbrueckii and L.fermentum supernatants and Fluconazole did not show a synergic effect on the biofilm formation and dispersion of the three strain, and the combination of fermentation supernatants and Fluconazole was not as effective as fermentation supernatants alone. The dispersion effect of L.fermentum fermentation supernatant on biofilm was better than that of L.delbrueckii fermentation supernatant. L.fermentum fermentation supernatant inhibited the initial adhesion of C.albicans. Conclusion L.delbrueckii and L.fermentum fermentation supernatants alone can interfere with the formation and dispersion of Fluconazoleresistant C.albicans biofilm; there is no synergistic effect when Fluconazole was used in combination. This inhibitory effect on drug resistant C.albicans biofilm may be related to the inhibition of mycelia formation and initial adhesion.